Cyanotis arachnoidea C. B. Clarke is a Commelinaceae herb, which is the earliest resource plant found to be rich in β-ecdysterone. Therefore, we generally use this plant for the extraction process.
The extraction method of β-ecdysterone is as follows:
1. Take the dew grass as the starting material, after crushing, add water to boil for 1 hour, extract with water 3 times, combine the extracts, add clarifier (1% acetic acid solution of 1% clarifier) while hot, and precipitate out After, add clarifying agent (5% aqueous solution of clarifying agent) at 50 ~ 80 ° C, filter, concentrate, extract four times with methanol-ethyl acetate (2:8), add 10 ml of water, remove the organic solvent, excessively large The porous resin was first eluted with water, then eluted with a gradient of water-80% ethanol (9:1~2:8), and collected the water-80% ethanol (5:5~6:4) eluate (β-ecdysteroid). ketone component), concentrated under reduced pressure to dryness, and the residue was crystallized with a small amount of methanol to obtain β-ecdysterone.
2. Using dew grass as the starting material, after crushing, heating, and refluxing with 75% ethanol for extraction, recovering the ethanol to obtain a concentrated solution, extracting with ethyl acetate, passing the extract through neutral alumina column chromatography, and washing with chloroform-methanol gradient The eluted fractions of chloroform-methanol (4:1) were collected, concentrated, and the residue was recrystallized from methanol-ethyl acetate to obtain β-ecdysterone finished product.
Through a comprehensive comparison of the above two methods, we take 1 as a reference, and the methods are as follows:
Take the dew grass as the starting material, after crushing, add water to boil for 1 hour, extract with water 3 times, combine the extracts, add 1% acetic acid solution of 1% ZTC1+1 clarifying agent while hot, and add 1% acetic acid solution after precipitation. The aqueous solution of the ZTC1+1 clarifying agent of 5% is filtered, and the filtrate adds the acetic acid solution of the ZTC1+1 clarifying agent of 1% of the ZTC1+1 clarifying agent at 50～80° C. After precipitation is arranged, at 50～80° C. Add 5% aqueous solution of ZTC1+1 clarifier, filter, concentrate the filtrate, extract the residue four times with methanol-ethyl acetate (2:8), concentrate the extract, remove the organic solvent, add 10ml of water to the residue, Excessive macroporous resin, eluted with water first, then gradient elution with water-80% ethanol (9:1~2:8), and collected water-80% ethanol (5:5~6:4) eluent (β- Ecdysterone component), concentrated to dryness under reduced pressure, and the residue was crystallized with a small amount of methanol to obtain β-ecdysterone.
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